Characterisation of an aptamer against the Runt domain of AML1 (RUNX1) by NMR and mutational analyses

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Characterisation of an aptamer against the Runt domain of AML1 (RUNX1) by NMR and mutational analyses

Since the invention of systematic evolution of ligands by exponential enrichment, many short oligonucleotides (or aptamers) have been reported that can bind to a wide range of target molecules with high affinity and specificity. Previously, we reported an RNA aptamer that shows high affinity to the Runt domain (RD) of the AML1 protein, a transcription factor with roles in haematopoiesis and imm...

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The core binding factor (CBF) heterodimeric transcription factors comprised of AML/CBFA/PEBP2alpha/Runx and CBFbeta/PEBP2beta subunits are essential for differentiation of hematopoietic and bone cells, and their mutation is intimately related to the development of acute leukemias and cleidocranial dysplasia. Here, we present the crystal structures of the AML1/Runx-1/CBFalpha(Runt domain)-CBFbet...

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ژورنال

عنوان ژورنال: FEBS Open Bio

سال: 2018

ISSN: 2211-5463

DOI: 10.1002/2211-5463.12368